NIH3T3 fibroblast produces PtdIns(3,4,5)P3 in response to PDGF.
Didn't you always want to move to Southern California to make movies?
Our lab studies the phosphoinositides, a group of signaling molecules involved in growth factor signaling, membrane trafficking, and probably many more functions. We are taking several approaches to identify novel functions for this interesting group of signaling molecules. One approach uses fluorescence microscopy of living mammalian cells expressing green fluorescent protein reporters for each of the phosphoinositides. Another approach involves a screen of the entire Drosophila proteome to identify novel phosphoinositide-binding proteins. Both approaches have yielded new and unexpected functions for the phosphoinositides. For example, we have identified an interesting role for PI(4,5)P2 at the cleavage furrow during mitotic cytokinesis. We have many projects to study our new phosphoinositide binding proteins (including their role in cytokinesis) and to develop new approaches to study phosphoinositide function. Because of the broad role for phosphoinositides in the cell, we are always ready to delve into new areas of cell biology as our research leads us.
General strategies to study phosphoinositides
• Live cell imaging of phosphoinositides
Changes in the level or sub-cellular localization of a phosphoinositide can provide a clue to new functions.
PtdIns(4,5)P2 accumulates at the cleavage furrow during cytokinesis.
You can also find research summaries for our lab at the following sites:
Seth Field's Lab
Studying Phosphoinositides at UCSD
© 2005-2014 Seth Field